Mesenchymal stromal cell secretome in preserving myocardial transcriptome profile following ex vivo cold storage. Mesenchymal stromal cell secretome in preserving myocardial transcriptome profile following ex vivo cold storage

Deep RNA sequencing analysis revealed that compared to control (no cold storage/ischemia), 6h-cold storage led to 266 differentially expressed genes, many of which were implicated in modulating mitochondrial performance, oxidative stress response, myocardial function, and apoptosis. Either bone marrow- (BM) or adipose tissue-derived (Ad) mesencymal stromal cell conditioned medium (MSC-CM) restored these gene expression towards control. Overall design: Total RNAseq was performed from the RNA isolated from mouse hearts without storage or stored in cold presrevation soultion +/- BM-MSC CM or Ad-MSC CM for 6 hours.