Intersecting patterns, kinetic mechanisms and kinetic constants of VcIPK and VcIIPK.

Intersecting patterns were taken from the double reciprocal plots of the initial velocity data. The data shown in Fig 6 were globally fitted (nonlinear regression Origin versión 7.0) to the equation describing a rapid equilibrium random order mechanism v = Vmax [A] [B]/ (KaKb + Ka [B] + Kb [A] + [A] [B]), where v represents the initial velocity, A is PEP3-, B is ADP-M2+ and Ka and Kb are the Michaelis-Menten constants for PEP and ADP-M2+, respectively. Standard deviation values are shown. Catalytic efficiency values, kcat/Km (M1s-1) are expressed in log form.