HOXA13 Cut & Run of Human Melanoma Cells

The goal of the experiment was to determine HOXA13 binding loci in human melanoma cells to infer downstream targets of a HOXA13 positional program. Overall design: We performed Cut&Run for the melanoma cell lines SKME-1088, SKMEL-1176, and SKME-1206. For Cut&Run we used 100'000 cells per condition and it was performed as described in110. We used antibodies against HOXA13 (Invitrogen, #PA5-76440, 1:100), H3K27ac (Active Motiv, #39034, 1:100), IgG (abcam, #ab6709, 1:100) and we added a “no antibody” condition as additional negative control.