Identification of novel protein-binding ncRNAs in mouse by generation of cDNA libraries from ribonucleo-protein particles

We have developed a novel method for identification of novel ncRNAs, which involves the generation of cDNA libraries from size-fractionated RNPs. We applied the RNP method to mouse brain cells as a model system for higher eukaryal organism and subjected the cDNA library to deep-sequencing. Bioinformatical analysis of cDNA sequences revealed hundreds of novel ncRNA candidates. In addition, known classes of ncRNAs were identified, all reported to form RNPs, such as miRNAs, snRNAs or snoRNAs.