Transcriptome analysis of the effect of RAD52 knockout on olaparib response in BRCA2-deficient, olaparib-resistant murine ovarian cancer cells (ID8-OR)

This study investigates the role of RAD52 in PARP inhibitor-resistant BRCA2-deficient ovarian cancer, with a particular focus on identifying compensatory DNA repair pathways. To explore RAD52-regulated transcriptional programs, we used CRISPR/Cas9 to generate RAD52 knockout clones in the PARP inhibitor-resistant, BRCA2-deficient mouse ovarian cancer cell line ID8-OR. Scrambled guide RNA-transduced cells served as controls. Both RAD52 knockout and control cells were treated with either olaparib or vehicle. RNA was extracted from all samples (n=3 per group; four groups total) for transcriptome analysis. This dataset provides a valuable resource for investigating RAD52-dependent gene expression and alternative DNA repair mechanisms in the setting of PARP inhibitor resistance. Overall design: Gene expression profiling of RAD52 knockout and control ID8-OR cells. Cells were treated with 1 µM olaparib or vehicle for 24 hours and collected in biological triplicate.