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In planta deglycosylation improves the SARS-CoV-2 neutralization activity of recombinant ACE2-Fc

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NIAID Data Ecosystem2026-05-01 收录
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The ectodomain of human ACE2-Fc was transiently expressed in leaves of Nicotiana benthamiana plants and protein A and SEC purified. SEC-purified ACE2-Fc was sequentially digested with chymotrypsin and trypsin and the glycopeptides were subjected to LC-ESI-MS analysis using the LC-OTMS, Orbitrap Exploris 480 from Thermo Scientific.

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2023-04-18
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