Molecular mechanisms of increased survival of Vibrio cholerae flrA mutant in amoeba

Mutation in flagellar transcriptional regulatory gene (flrA) result in increased V. cholerae growth and intracellular survival in the amoeba host Acanthamoeba castellanii. To identify molecular mechanisms behind the increased survival and growth of the flrA mutant transcriptomics assay was done. To compare transcriptomes of the flrA mutant during amoeba predation RNA-seq and transcriptomics analysis was performed. The V. cholerae A1552 wt strain and its isogenic flrA mutant were used to infect A. castellanii at 100 MOI.