Bojungikgi-tang improves skin barrier function and immune response in atopic dermatitis mice fed a low aryl hydrocarbon receptor ligand diet

To identify the effects of BJIKT on skin homoestasis via skin barrier, AhR target genes and the immune response profile in AD mice fed a low-Ahr ligand diet. Overall design: The low Ahr ligand diet (ENVIGO [#TD130959]) group is provided with Ahr ligand free diet paper beddings (#SSP0001, ALPHA-dri, Shepherd Specialty Papers, Watertown, TN). A low Ahr ligand diet was carried out for 12 weeks. After 2 weeks of a low Ahr ligand diet, atopic dermatitis (AD) was induced with 2,4-dinitrochlorobenzene (DNCB), and Bojungikgi-tang (BJIKT, 1g/kg/day in water) was administered by oral gavage daily at the same time. To induce AD, dorsal skin was applied twice a week with 1% DNCB mixed in acetone/olive oil (3:1). Comparative gene expression profiling analysis of Skin RNA-seq data obtained from C57BL/6 mice in Control group, Low Ahr ligand diet group, the AD mice fed a Low Ahr ligand diet, BJIKT treated AD mice fed a Low Ahr ligand diet