Gene expression data from RT112 following FGFR3 knock-down using FGFR3 siRNAs

To better understand the molecular mechanisms underlying altered-FGFR3 oncogenic activity in bladder carcinomas, we made use of RT112 cell lines, which were derived from a human bladder tumor and endogenously expressed the FGFR3-TACC3 fusion protein, the growth and transformation of these cell lines being dependent on activated-FGFR3 activity. We conducted a gene expression analysis using Affymetrix DNA arrays in this cell line treated or not with FGFR3 siRNAs. RT112 cells were transfected or not with siRNA FGFR3. mRNA was extracted after 40h and gene expression analysis was assessed using the HG-U133 Plus 2.0 array (Affymetrix) array