Topoisomerase II drug cleavage sites (no formaldehyde)

TOP2A and TOP2B bind and cleave genomic DNA. These enzymes are targeted by some anti-cancer drugs that stabilise the enzyme complexes on the DNA. Whole genome TOP2 ChIP-seq was carried out on human KG-1 cells in the presence and absence of the topoisomerase targeting drugs Etoposide and Mitoxantrone in the absence of formaldehyde. Overall design: Whole genome TOP2 ChIP-seq was caried out on human KG-1 cells in the presence (60 minute exposure) and absence of the topoisomerase targeting drugs Etoposide or Mitoxantrone. Input control samples were also sequenced for background detection and comparison.