Burden- driven feedback control of gene expression. Burden- driven feedback control of gene expression

RNAseq analysis was performed on 90 E. coli samples in order to identify the transcriptional cellular response to the burden of heterologous gene expression Early responsive promoters that are upregulated in response to burden were identified. The htpG1 early responsive promoter was selected to guide the activation of a burden- responsive feedback controller able to decrease heterologous gene expression while rescuing cellular capacity and growth Overall design: E. coli DH10B/MG1655, previously modified to bear a GFP expressing cassete integrated in the genome [Ceroni et al Nat Methods 2015], were transformed with 4 synthetic constructs and their empty plasmids. RNAseq analysis was performed at 15 and 60 min after induction of gene expression to capture the very first response to burden in these systems.