Isoform-resolved transcriptome of the human preimplantation embryo

This SuperSeries is composed of the SubSeries listed below. RNA sequencing data was generated from a total of 73 human embryos across six pre-implantation stages: 13 zygotes (1C), 13 2-cell (2C) embryos, 16 4-cell (4C) embryos, 15 8-cell (8C) embryos, 3 morulae and 13 blastocysts. For each embryo, mRNA was extracted and converted to full-length cDNA, and used to generate Illumina RNA-Seq data, generating an average of 58.6 million paired end reads per sample. The same cDNA samples were used for SMRT-seq, pooling together low-yield samples to obtain enough mass for long-read sequencing. This resulted in a total of 10,139,308 full-length nonchimeric (FLNC) reads across 10 Sequel II SMRTcells, with an average cDNA length of 1,332 bp.