Gene expression profiling of human colorectal cancer cell lines [Affymetrix]

We observed human colorectal cancer cell lines obtained resistance under treatment, and resistant cells switched back to sensitive state when the treatment is withdrawn. To understand gene expressions change during this process, we performed Affymetrix experiments for parental, R20 (resistant), and W20 (withdrawal treatment) clones for three colorectal cancer cell lines. Parental is the original cell line, and R20 are the cells survived 20 cycles of cetuximab and natural killer cells treatment, W20 cells are the R20 cells cultured without treatment for another 20 cycles.