In Vivo ectopic expression of OVOL2 and HNF1A in the context of PDA

We tested whether ectopic expression of OVOL2 and HNF1 either individually or in combination—the gene pairs inducing the most efficient MOS ? GLS transdifferentiation in vitro—could recapitulate MOS ? GLS cell state transdifferentiation in vivo Overall design: Plasmids containing ORF constructs for OVOL2, HNF1A, and the OVOL2-HNF1A as well mCherry (identical to those used for the in vitro assays) were lentivirally transduced into morphogenic KP4 cells. Puromycin-selected cells were then pooled and injected into athymic mice (n = 3). Animals were sacrificed at 14 days following injection and mCherry positive cells were scRNA-seq profiled