High diversity but limited expression of biosynthetic gene clusters in a peatland microbial community

# Readme for data and code for Human ZR, Hoover R, Chan C, Küsel K, Wegner CE (2026 ). High diversity but limited expression of biosynthetic gene clusters in a peatland microbial community This repository contains the R scripts used to generate figures and results presented in the paper. Some additional data are also provided. All R scripts are provided as R Markdown, and a matching ZIP folder is available with the input files used in each analysis. Fig 3 was made in iTOL and used no R scripts or other code. Data and code for main figures are listed below. We provide R scripts and input files in the respective .zip files, and R markdown as HTML files with the executed scripts. 01_Fig_1AB.html - Markdown for Fig 1 A and B 02_BGC_fragmentation_analysis.hmtl - Markdown for BGC fragmentation analysis, python scripts used to collect data from antiSMASH GBKs in .zip 03_Fig2ab_GCF plots.html - Markdown for Fig 2 A and B 04_Fig2cd_BGC_density.html - Markdown of executed code for Fig 2 C and D 05_Fig4abcd_contig_expression.html - Markdown of exectuted code for Fig. 4 A-D 06_Fig5abcd_MAG_expression.html - Markdown of executed code for Fig 5 A-D 07_Fig6abc_FigS12.html - Markdown of executed code for Fig 6 A-C and Fig 12A and B Code and Input - BGC Fragmentation - Code used for BGC fragmentation analysis Code and Input Fig 2 - Input data and R scripts for all panels of Figure 2 Code and Input Fig 4 - Input data and R scripts used to produce all panels of Figure 4 Code and Input Fig 5 - Input data and R scripts used to produce all panels of Figure 5, including all MAG BGC-expression analyses Code and Input Fig 6 - Input data and R scripts used to produce all panels of Figure 6, and includes the associated supplementary figure Fig 3 was made in iTol and thus no code is provided # Other data: Fen_gbks.zip -> antiSMASH GBK files for all BGCs produced in this study ALL_MAGs_USED.zip -> Fasta files for all MAGs used in this study BiG_SCAPE_out.zip -> Output of BiG-SCAPE analysis of all GBKs from this study - note these data are also incorporated in fig 2 A and B above BGC_information.xlsx - Predicted product class- Taxonomic assignment- BGC identifiers- Corresponding file names