Behavioral estimates of mating success corroborate genetic evidence for pre-copulatory selection

In promiscuous species, fitness estimates obtained from genetic parentage may often reflect both pre- and post-copulatory components of sexual selection. Directly observing copulations can help isolate the role of pre-copulatory selection, but such behavioral data are difficult to obtain in the wild and may also overlook post-copulatory factors that alter the relationship between mating success and reproductive success. To overcome these limitations, we combined genetic parentage analysis with behavioral estimates of size-specific mating in a wild population of brown anole lizards (Anolis sagrei). Males of this species are twice as large as females and multiple mating among females is common, suggesting the scope for both pre- and post-copulatory processes to shape sexual selection on male body size. Our genetic estimates of reproductive success revealed strong positive directional selection for male size, which was also strongly associated with the number of mates inferred from parenta..., We studied an island population of brown anole lizards (Anolis sagrei) in the Guano Tolomato Matanzas Natural Estuarine Research Reserve in northern Florida (29°37′53′′ N, 81°12′ 46′′ W) using procedures approved by the University of Virginia Animal Care and Use Committee (protocol 3896). To assay the reproductive success of males, we sampled all adults and juveniles of the population at four different times during the breeding season (March, May, July, and October) in 2019. This population has been the focus of a long-term mark-recapture study since 2015, such that most adults in the 2019 breeding season were first captured, marked, and genotyped as juveniles in 2017 or 2018. We measured the snout-vent length (SVL, nearest 1 mm) and body mass (nearest 0.01g) of all individuals prior to releasing them at their exact site of capture the following day. We extracted DNA from adults captured in the population and used SNPPIT 2.0 (Anderson 2012) to assign genetic parentage. At two points in ..., , # Behavioral estimates of mating success corroborate genetic evidence for pre-copulatory sexual selection in the wild \[Access this dataset on Dryad](10.5061/dryad.41ns1rnmn) The findings in this paper are drawn from a mark-recapture and genotyping study carried out on a focal island population of brown anole lizards along the intra-coastal waterway in Guano Tolomato Matanzas Natural Estuarine Research Reserve in Florida. Although animals in this population have been tracked between 2015 and 2019 part from a long-term ongoing study, this dataset primarily focuses on the adult male population present on the island in 2019, their reproductive fitness, and pre-and post-copulatory components of fitness. We asked if selection due to total reproductive success on a measure of body size (here, body mass) is primarily due to higher mating success (pre-copulatory selection) or is primarily due to competitive fertilization success (post-copulatory success). We also tested alternate hypotheses f...在混交物种中，通过遗传亲权分析得到的适合度估计值，通常可同时反映性选择的交配前与交配后组分。直接观察交配行为有助于分离交配前选择的作用，但这类行为数据在野外难以获取，且可能忽略那些改变交配成功率与繁殖成功间关系的交配后因素。为克服上述研究局限，本研究结合遗传亲权分析与体型特异性交配行为的野外估测，针对野生褐安乐蜥（Anolis sagrei）种群开展研究。该物种雄性体型为雌性的两倍，且雌性多次交配现象普遍，表明交配前与交配后过程均可能参与塑造雄性体型相关的性选择。我们的繁殖适合度遗传估测结果显示，雄性体型存在显著的正向定向选择，且该选择与通过亲权分析推断的交配伴侣数量密切相关…… 本研究的研究对象为佛罗里达州北部瓜诺·托洛马托·马坦萨斯天然河口研究保护区内的褐安乐蜥（Anolis sagrei）岛屿种群（地理坐标：29°37′53″ N，81°12′46″ W），所有实验流程均通过弗吉尼亚大学动物护理与使用委员会审批（协议编号：3896）。为测定雄性繁殖适合度，我们于2019年繁殖季的4个时间节点（3月、5月、7月、10月）采集该种群全部成体与幼体样本。该种群自2015年起便被纳入长期标记重捕研究，因此2019年繁殖季的绝大多数成体，均在2017或2018年以幼体身份完成首次捕获、标记与基因分型。我们测量了所有个体的吻肛长（SVL，精度至1 mm）与体质量（精度至0.01 g），并于次日将个体放归至捕获时的精确位点。我们从捕获的成体样本中提取基因组DNA，并使用SNPPIT 2.0（Anderson等，2012）完成遗传亲权鉴定。相关研究将在两处节点展开…… # 交配成功率的行为估测验证了野外交配前性选择的遗传证据 [访问Dryad平台获取本数据集](10.5061/dryad.41ns1rnmn) 本研究的结论源自一项针对褐安乐蜥目标岛屿种群的标记重捕与基因分型研究，该种群分布于佛罗里达州瓜诺·托洛马托·马坦萨斯天然河口研究保护区内的沿岸水道沿线。尽管该种群个体自2015至2019年已被纳入长期持续跟踪研究，但本数据集主要聚焦2019年岛上的成年雄性种群、其繁殖适合度，以及适合度的交配前与交配后组分。我们旨在探究：基于体质量这一体型指标的总繁殖适合度所介导的选择，究竟主要源于更高的交配成功率（交配前选择），还是主要源于受精竞争成功率（交配后成功）。本研究同时检验了替代假说……