Determination of immune responses in mucosal lymphoid tissues following infectious laryngotracheitis virus (ILTV) eye drop and vent brush vaccination of chickens

Eye drop (ED) and vent brush (VB) application of live infectious laryngotracheitis virus (ILTV) vaccine provides protective immunity against disease, but little is known regarding the immune responses following application. This study compared the gene expression of immune markers associated with VB and ED ILTV vaccination in the conjunctiva, trachea and cloaca at 3, 5, 7 and 10 days post-vaccination (dpv) and 3, 5 and 7 days post-revaccination (dprv). The relative gene expression of markers associated with inflammatory responses and their regulation (CCL4, CCR5, IL2, IL4, IL6, IL17C, IFNG), pathogen recognition receptors (TLR2-2, TLR4, TLR7) and cell surface markers (CD4, CD8A, CD14, CD80) was evaluated by RT–PCR after ED or VB vaccination and revaccination using the alternate route (ED/VB (primary/booster), VB/ED). There was increased expression of inflammatory markers at vaccination sites following primary vaccination. In the VB group, there was increased expression of IL6 and IFNG in cloaca (3 dpv) and IL2 in the conjunctiva (7 dpv) compared to the ED group (P RESEARCH HIGHLIGHTSILTV vaccination elicited inflammatory responses at the vaccination sites (conjunctiva and cloaca).Conjunctival or cloacal vaccination elicited similar inflammatory responses in the trachea after 3 and 5 days, respectively.Revaccination by a different route stimulated similar immune responses in conjunctiva, cloaca and trachea. ILTV vaccination elicited inflammatory responses at the vaccination sites (conjunctiva and cloaca). Conjunctival or cloacal vaccination elicited similar inflammatory responses in the trachea after 3 and 5 days, respectively. Revaccination by a different route stimulated similar immune responses in conjunctiva, cloaca and trachea.