16S amplicon sequence analysis of human gut microbiome in patients with Inflammatory bowel disease (IBD)

Abnormal composition of gut microbiota, termed dysbiosis, has been implicated in pathogenesis of inflammatory bowel diseases (IBD). In order to investigate associations between dysbiosis and fecal metabolites, we performed 16S ribosomal RNA (rRNA) amplicon sequence analysis of fecal samples from 23 Japanese healthy subjects, 30 ulcerative colitis patients and 25 Crohn's disease patients. The V3 and V4 regions of the 16S rRNA gene were sequenced with a MiSeq 600 cycle v3 kit (Illumina).