Mass spectrometry data profiling whole cell lipid extractions from Mycobacterium tuberculosis H37Rv and strains mutated in Rv3376

Mass spectrometry data files of whole cell lipid extracts from strains of Mycobacterium tuberculosis with engineered replacement of the gene Rv3376 and the non-mutated parental strain H37Rv. Biological triplicate samples from each strain were profiled to assess terpene nucleoside biosynthesis. These data were analyzed in the manuscript, “Mycobacteria that cause tuberculosis have retained ancestrally acquired genes for the biosynthesis of chemically diverse terpene nucleosides” (https://doi.org/10.1371/journal.pbio.3002813) and were included in analyzed form (DOI 10.5281/zenodo.8096634). FIles include mass spectrometry data in centroided mzXML format, instrument metadata, and an annotated sample list.