Sanger sequence chromatograms of 17KDa and gltA Rickettsia genes demonstrating double peaks

A multilocus sequencing study of Torix group Rickettsia in arthropods providing evidence of co-infection with Rickettsia. During Sanger
chromatogram analysis, double peaks were occasionally found at third codon
sites from protein coding genes. This pattern was observed in 6/10 Philotarsus
californicus individuals and in one member of each of the Psilidae,
Sciaridae, Chironomidae and Diapriidae. Where double peaks were
observed, this was found consistently across markers within an individual
specimen. This pattern indicates co-infecting Rickettsia strains in
hosts is a widespread phenomenon of the Torix group.