Ebag9 defines a regulatory checkpoint for CD8+ T cell memory formation responding to non-infectious stimuli

We examined the role of cytolytic strength on T cell fate decisions made by functionally competent CTLs under non-inflammatory priming conditions. Employing the HY-specific miHAg system in conjunction with non-inflammatory priming conditions, we demonstrate a higher cytolytic efficacy despite unchanged effector frequencies in the primary response. In Ebag9-/- mice, an expanded memory population was obtained for anti HY- and anti SV40 T antigen-specific CD8+ T cells. CD8+ memory commitment was analyzed by single-cell RNA-seq in Ebag9-deficient mice that exhibit an amplified secretion of granzyme A and an augmented allogeneic and tumor cell lysis.