Su(H) ChIP-on-chip of Notch-stimulated overgrowing Drosophila melanogaster wing imaginal discs

Aim: Su(H) chromatin occupancy profiling by ChIP on larval wing imaginal discs of Drosophila melanogaster to study the cooperation between Notch activation and loss of epithelial polarity (scrib mutation) during neoplastic growth. Results: The combination of Notch activation and scribble mutation (NS) does not lead to a general redeployment of Su(H) binding as compared to individual conditions (Notch only (N), and scrib mutation only (S)) Duplicates of pooled dissected 3rd instar larva wing imaginal discs. All dissections at day 6 after egg laying for the following conditions: activated Notch (N), polarity deficient (scrib1 mutant, S), activated Notch and polarity deficient (NS)