Cellular level 3D reconstructed volumes at 1µm resolution within the BigBrain occipital cortex

This dataset contains two 6x6x6 mm³ volumes sampled from the occipital cortex (areas V1 and V2) of a human postmortem brain. The volumes were acquired by rescanning the original histological sections of the BigBrain model at an isotropic resolution of 1µm. The sections were linearly 3D reconstructed by aligning matched pairs of bisected cells, which were identified from congruent point constellations. Subsequently, both reconstructed stacks were anchored into the 20µm 3D BigBrain space using a 3D affine transformation. Each reconstructed volume is provided as a multipage (Big)Tiff file at multiple resolutions (1-32µm) which can be interpreted by most biomedical image viewers. Additionally, the volumes can be explored interactively online, superimposed on the BigBrain model, using the links provided. Direct integration with the EBRAINS atlas viewer is in progress.