Single-cell transcriptome dynamics in stabilized pituitary gonadotrope cells [smartseq_pituitary]
收藏干细胞与再生医学数据中心2022-02-20 更新2024-03-06 收录
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http://data.iscr.ac.cn/Article?id=343d3f75b0f825a743f78b25573bb979
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资源简介：
Studying dynamic transcripts in single cells (SC) requires large numbers of timed samples. We report an easy to use protocol to stabilize RNA in intact SCs without perturbing transcriptional patterns, and demonstrate its applicability for SC transcriptome assays with cells and tissue. We identify a gene-specific hierarchical pattern of all-or-none transcript induction elicited by different concentrations of pulsatile hormone stimuli in pituitary gonadotropes.
提供机构：
Icahn School of Medicine at Mount Sinai
创建时间：
2022-02-20
