microarray analyses. Homo sapiens

Two types of renal proximal tubular cells were used: HK-2, which is a cell line, and RPTECs, which are primary cells. Using TGF-b1 (10 ng/mL) and hypoxia (1% O2) for stimulation, lncRNAs for which expression was increased in common under four conditions (HK-2+TGF-b1 or hypoxia, and RPTEC+TGF-b1 or hypoxia) compared with controls (HK-2 cells and RPTECs, respectively) were extracted. Significantly differentially expressed lncRNAs between treatment and control groups were identified based on p-value (1.5) filtering. To evaluate sets of related genes that could be systematically altered under all four conditions, we conducted a study of functional pathway gene sets by analyzing the microarray dataset using GSEA. GSEA application, version 4.1.0, was used to analyze the microarray data. Gene sets for GSEA were taken from hallmark gene sets as Gene Symbols, version 7.4