Single cell RNA-sequencing of lungs of Ifngr1-/-Rag-2-/- mice. Single cell RNA-sequencing of lungs of Ifngr1-/-Rag-2-/- mice

We discovered Ifngr1-/-Rag-2-/- mice spontaneously develop severe pulmonary fibrosis (PF), and divided the progression process of PF in this strain into 3 phases; the pre-disease phase (until 12 weeks old; intact), the onset phase (from 12 to 18 weeks old; characterized by inflammations), and the chronic phase (after 18 weeks old; characterized by collagen deposition and obvious clinical symptoms). For the purpose of understanding the transcriptome dynamics of each phase, we performed single-cell RNA-Seq analysis on the whole lung cells from Rag-2-/- mice and Ifngr1-/-Rag-2-/- mice of different ages, including the pre-disease (7 weeks old), the onset (12 and 16 weeks old), and chronic phases (19 and 24weeks old). Overall design: The whole lungs of Ifngr1-/-Rag-2-/- mice (7, 12, 16, 19, and 24 weeks old), and Rag-2-/- mice (18 weeks old; control) were isolated and dissociated, and then single-cell libraries of the whole lung cells were generated using the Chromium Single Cell 3’ v2 Reagents kit (10x Genomics).