allvalues_Calluna.xlsx

The published images were analyzed and the obtained values were summarized in the Excel files.For measurement of the anatomical parameters, the software Optimas 6.5 (Media Cybernetics, L.P. Optimas, Rockville, MD) was used.Values were obtained as follows: within a 0.00316 mm2 area of xylem the number of tracheae and tracheids (ntt), the outer diameter (dtt), and the cell wall thickness of those xylem elements (tcw) were measured. From these values, the ratio of cell wall thickness to cell diameter (r = tcw/dtt) was calculated. Additionally, the whole xylem area in a cross section (Ax) was determined by using dx and assuming a circular shape of the vascular tissue in a cross section. If pith tissue was present, the area of the pith tissue was subtracted from the xylem area. By knowing Ax and ntt the total number of conducting xylem units in the cross sectional area of xylem (n) was calculated. There were only tracheids found in the transition zone (Z3) and the basal part of the pedicel (Z4), whose lengths (ltr) were measured.本研究对已发布的图像进行分析，并将所得测量值汇总于Excel文件中。针对解剖参数的测量，采用Optimas 6.5软件（Media Cybernetics, L.P. Optimas，美国马里兰州罗克维尔）完成。具体测量流程如下：在面积为0.00316 mm²的木质部区域内，分别统计导管与管胞的数量（ntt）、测量该类木质部结构的外径（dtt）与细胞壁厚度（tcw）。基于上述测量值，进一步计算得到细胞壁厚度与细胞直径的比值（r = tcw/dtt）。此外，通过测量直径dx并假设横切面中的维管组织呈圆形，确定横切面内的整体木质部面积（Ax）；若横切面中存在髓组织，则从木质部总面积中扣除髓组织的面积。结合Ax与ntt的数值，可计算得到横切面木质部区域内的总输导木质部单元数量（n）。在过渡区（Z3）与花梗基部（Z4）仅观测到管胞，并对其长度（ltr）进行了测量。