Single-molecule and bulk fluorescence and biolayer interferometry on the NAD-II riboswitch and its interactions with NMN

The Class II NAD+ riboswitch is a bacterial RNA that binds ligands containing nicotinamide. Herein, we report a fluorescence and biolayer interferometry study of riboswitch interactions with β-NMN. The results reveal a shift in the prevalence of a pseudoknot structure in the presence of ligand and Mg2+., Bulk fluorometry, single-molecule microscopy, gel electrophoresis, biolayer interferometry data on the NAD-II riboswitch., , # NAD+-II riboswitch

[https://doi.org/10.5061/dryad.4b8gthtnw](https://doi.org/10.5061/dryad.4b8gthtnw)

## Description of the data and file structure

The NAD+-II riboswitch was studied using bulk and single-molecule fluorescence and biolayer interferometry.

### Files and variables

#### File: NADII-data.zip

**Description:** 

`BLI_P1a` contains biolayer interferometry traces for P1a-modified riboswitch and fits.

* \"assay_full\" contains times and corresponding datapoints for all samples.
* 20240729_whole_align_assoc contains contains times and corresponding datapoints with the first datapoint in the association phase shifted to match for all samples.
* 1to1fitassoc and 2to1fit_assoc contain 1:1 and 2:1 fits to the association phases of P1a-Cy5 RNA in 20 mM MgCl2. This data was exported from the software of a Gator Bio BLI instrument and can be opened in any program that reads text or tables.

`BLI_U14` contains BLI traces and fits for U14-modified riboswitch. \"assay_full\" contai...