Properties of the Crf2 specific scFv antibody clones.
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Abbreviations: n.d. - not determined,1Epitopes were concluded from scFv binding to denatured Crf2 in Western immunoblots (linear epitope) or exclusive binding to native antigen on A. fumigatus in immunofluorescence microscopy (conformational epitope).2Serum half life of the scFvs were determined by incubation in PBS supplemented with human serum at 37°C.3Antigen detection limit of the scFv clones was measured in ELISA. Streptavidin was used for capturing biotinylated Crf2 without denaturation. Detection was performed with the scFv clones.
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2015-12-02
