Endothelial cell-specific reduction of heparan sulfate suppresses glioma growth in mice. Endothelial cell-specific reduction of heparan sulfate suppresses glioma growth in mice

we examined how HS-reduced vascular endothelial cells are involved in the proliferation of glioma by using mice in which Ext-1 expression is specifically suppressed in vascular endothelial cells. Overall design: We prepared mouse glioma (GL261) cells. We transplanted the cells to wild type (WT) and Ext1 flox/flox; VE-Cre mice (N=4 each). We removed the developing tumors from mouse brain 14 to 21 days later and extracted RNA from whole tumors. Finally, we perfomred RNA microarray analysis.