Gene expression changes in Laccaria bicolor mycelium grown under different nutritional conditions

Illumina technology was used to generate mRNA profiles of Laccaria bicolor mycelium grown under different nutrien conditions. Total RNA was extracted.TruSeq mRNA Stranded libraries were constructed and and sequenced (2 x 150 bp Illumina HiSeq3000) at the Genotoul sequencing facilities (Toulouse, France). Raw reads were trimmed for low quality (quality score 0.05), Illumina adapters and sequences shorter than 15 nucleotides and aligned to the L. bicolor v2 reference transcripts available at the JGI database https://mycocosm.jgi.doe.gov/Lacbi2/Lacbi2.home.html using CLC Genomics Workbench v8. Overall design: mRNA profiles of Laccaria bicolor grown under different nutrient conditions were generated by Illumina HiSeq 3000 (2x150bp). Three biological replicates were sequenced.