Evidence of morphological divergence and reproductive isolation in a narrow elevation gradient

Elevation gradients generate different environmental conditions. This environmental differentiation can influence morphological adaptation, habitat isolation, reproductive isolation, and pollinator limitation in plants. Habitat differentiation and isolation often act first on phenotypic traits and then on genotype variation, causing genetic divergences between populations. We evaluated the effect of elevation on morphological traits, reproductive isolation, and pollinator limitation in Croton aff. wagneri in dry shrublands of inter-Andean valleys in Ecuador. We measured morphological traits of Croton at three elevations and carried out experimental pollination crosses between and within each population at different elevations to assess the degree of reproductive isolation and pollinator limitation. Morphological traits such as leaf thickness, plant volume, inflorescence length and inflorescence number were dissimilar between plants in different elevations. There was evidence of incipien...