Mapping the zebrafish developmental and aging atlas by single cell mRNA-seq

Single-cell mRNA sequencing (scRNA-seq) technologies are reshaping current cell-type classification system. In previous studies, we constructed the Mouse Cell Atlas (MCA) and Human Cell Landscape (HCL) to catalog all cell types by collecting scRNA-seq data. Howerver, the systematic study for organism-level dynamic changes of cellular states across zebrafish life span are still lacking. Here, we constructed the zebrafish cell Landscape covering different development periods using Microwell-seq protocol. The zebrafish cell landscape provides a valuable resource for studying cross-sepciess development, maturation and aging. Over 452,878 cells derived from zebrafish whole-body single-cell dissociation and profiling at the larval stage (21 day, 3 replicates, and 10 fish for each replicate) and adult (22 month, 6 replicates, 1 fish for each replicate).