Gene expression in F. tularensis subsp. tularensis SCHU P0, P5, and P9 strains.. Gene expression in F. tularensis subsp. tularensis SCHU P0, P5, and P9 strains.

The microarray analysis of Francisella tularensis subsp. tularensis strains SCHU P9 (virulent) and SCHU P0/P5 (attenuated) revealed significant differences in gene expression. The results showed that 19 genes were upregulated in the virulent SCHU P9 strain, while two genes, including ftt_0965c, were significantly downregulated Overall design: Francisella tularensis subsp. tularensis strains SCHU P9 (virulent) and SCHU P0/P5 (attenuated) were analyzed to screen for potential differences in gene expression. These strains were cultured on chocolate Eugon agar plates at 37°C for 2 days, with each strain being cultured in duplicate, providing two biological replicates per condition. After 48 hours of incubation, total RNA was extracted and quality-checked using a Bioanalyzer 2100. The microarray involved hybridizing the labeled RNA from these strains onto an Agilent microarray chip containing probes specific to the F. tularensis genome.