Summary of the BLASTn analysis of the different fragments considered to contain signature sequences essential for tracing micro-evolutionary events taking place within the CRL of GI3 in E coli O104:H4 draft genomes available in GenBank.

Fragment 3, spanning the Tn6029B transposon, was also used as a query sequence in BLASTn searches. Four genomes, including 2009EL-2050, have Tn6029B while 28 genomes appeared to have a substantial segment of the blaTEM-1 module as indicated by the different start sites of the input query in S3 Table. Our analysis indicates that a variant of Tn6029 is present in most of the EAHEC O104:H4 genomes. Unpublished genomes of Ec11–4984 (AHOU01000000), Ec11–4988 (AHOY01000000) and Ec11–4986 (AHOW01000000) submitted as supercontigs by the Broad Institute Genome Sequencing Centre for Infectious Diseases appeared to have copies of Tn6029B that are identical to those in 2009EL-2050 located within in a single contig.Summary of the BLASTn analysis of the different fragments considered to contain signature sequences essential for tracing micro-evolutionary events taking place within the CRL of GI3 in E coli O104:H4 draft genomes available in GenBank.