Replication of BgV in insects and vertebrates.

Insect and vertebrate cell lines were inoculated in triplicates with BgV and WNV at MOI 1 for 1h 30min, the inoculum removed and the cells washed before incubating the replenished cultures at 28°C or 37°C. The inoculated cell culture supernatants were harvested five days p.i., titrated on C6/36 cells and analysed by fixed-cell ELISA.—represents no replication, + represents a titre over 102 TCID50IU/mL, ++ represents a titre over 104 TCID50IU/mL, +++ represents a titre over 106 TCID50IU/mL. In a few instances, not all replicates were positive, in which case the titre represented is the average of the positive replicates only.