This dateset contains primary data and code used in the associated publication "Non-Canonical shedding of TNFa by SPPL2a is determined by the conformational flexibility of its transmembrane helix" . I
500 ns MD simulation of pure POPC membrane using Charmm-Drude polarizable force field. The system contains 128 POPC lipids, 51 NaCl, and 6400 SWM4 water molecules. The simulation have been performed
Fig 2k, l and n: Scatter plot of D/A versus the R/G values of endosomes in HEK 293T cells clamped at 6.3 pH and 0.1 mM [K+]. Fig 2m-p: 2-IM profiles of HEK 293T cells clamped at the indicated pH
6 ON Parasol Retinal Ganglion cells during full-field stimulation reported in Trong, P. K., & Rieke, F. (2008). Origin of correlated activity between parasol retinal ganglion cells. Nature neuroscienc
• This is a gliding motility assay using kinesin-1 and rhodamine labeled microtubules. • The buffer used was PEM (also known as BRB80) with the inclusion of 16551mM D2O. • Images were taken with an An