PDGFRb+ Mesenchymal Cells, but Not NG2+ Mural Cells, Contribute to Cardiac Fat

Understanding cellular origins of cardiac adipocytes (CAs) could offer important implications for the treatment of fat-associated cardiovascular diseases. Here, we performed lineage tracing studies using various genetic models, and found that cardiac mesenchymal cells (MCs) contribute to CAs in postnatal development and adult homeostasis. We found that PDGFRb+ cells are heterogenous, and revealed thatPDGFRa+PDGFRb+ double-positive periendothelial fibroblasts contribute to intramyocardial adipocytes. In contrast, SMCs and NG2+ pericytes did not contribute to CAs. We performed single cell RNA sequencing to compare the heterogenity.