DNA methylation analysis of NNMT overexpression and knockdown in stromal cells

Genome-wide DNA methylation profiling in response to overexpression or knockdown of NNMT in stromal cells. The Illumina MethylationEPIC array was used to profiled genome-wide methylation of normal fibroblasts expressing a control construct or NNMT overexpression construt and cancer-associated fibroblasts (CAFs) expressing an shCtrl or shNNMT construct. Two biological replicates of each experimental group were profiled following growth in 10 micromolar methionine growth media for 21 days. Bisulphite converted DNA from the 8 samples (4 experimental groups with two biological replicates per group) were hybridised to the Illumina InfiniumMethylationEPIC array.