RNA-seq analysis of Arabidopsis pen2 mutants

The circadian clock controls a plethora of physiological processes, including plant-pathogen interactions in plants. The circadian clock is composed of several transcription factors, including CIRCADIAN CLOCK ASSOCIATED1 (CCA1) and LATE ELONGATED HYPOCOTYL (LHY). CCA1 and LHY are Myb-like transcription factors, which bind to the evening element (EE) to regulate the expression of evening-expressed genes PSEUDORESPONSE REGULATOR 7 (PRR7), PRR5, and TIMING OF CAB EXPRESSION1 (TOC1). We have found that CCA1 contributes to nonhost resistance (NHR) to Pyricularia oryzae in Arabidopsis. To further assess the function of CCA1 in NHR, we conducted RNA sequencing (RNA-Seq) analysis of pen2 and pen2 CER6::CCA1 plants inoculated with P. oryzae in Arabidopsis.