Lipid metabolism associated with efficacy of metronomic capecitabine and camrelizumab in gastrointestinal cancer: an exploratory clinical trial

Lipid accumulation of skeletal muscle can be modulated after treatment of metronomic chemotherapy, while the detail molecular mechanisms are unclear. We used metronomic dose of 5-FU to treat C2C12 cell in vitro. RNA sequencing showed the expression of lipid metabolism related genes was changed after treatment. Overall design: Mouse myocytes C2C12 was treated with metronomic dose of 5-FU (5ug/L) for 24 hours, and PBS treatment was used as control. After treatment, cells were collected for RNA sequencing.