Native RNA sequencing in fission yeast reveals frequent alternative splicing isoforms

Spom_dRNA_cleanreads.fa.gz: Reads of dRNA after cleaning with Illumina reads and TranscriptClean. Spom_dRNA_sorted.bam: Alignment of clean reads to S. pombe genome. Spom_dRNA_Stringtie_transcriptome.gtf: Transcript assemblies and genomic coordinates generated by StringTie from dRNA reads. Spom_dRNA_Stringtie_transcriptome.fa: Sequences extracted from the annotation file generated by StringTie from dRNA reads. Spom_isoforms.gtf: Annotation file for genes with alternative isoforms not annotated in Pombase. Spom_novel_transcripts.gtf: Annotation file for novel transcripts not annotated in Pombase. Supplemental_Table_S9.tsv: Information of translated ORFs in ncRNAs. Spom_dRNA_5UTR_Stringtie.gtf: Genomic coordinates of new 5'UTR regions in S. pombe. Spom_dRNA_5UTR_Stringtie.fa: Sequences extracted from the annotation files of 5'UTR regions. Spom_dRNA_3UTR_Stringtie.gtf: Genomic coordinates of new 3'UTR regions in S. pombe. Spom_dRNA_3UTR_Stringtie.fa: Sequences extracted from the annotation files of 3'UTR regions. Supplemental_Table_S8.xlsx: Information on the transcripts identified with dRNA, new isoforms, novel transcripts and new UTR regions.