Single cell RNA sequencing of CD41+ and CD41- CD34+ HPC in model of RUNX1 insufficiency

To gain insights into the global and local transcriptomic changes underlying defects in adult RUNX1in HSPCs, we then performed scRNA SEQ on sorted CD41a- and CD41a+ HSPCs from cultures expressing NT or RUNX1-targeting lentiviral shRNA. Examination of gene expression in 12 sorted samples from control (3 CD41- and 3 CD41+) and RUNX1 haploinsufficient (3 CD41- and 3 CD41+) iHPCs based on CD42 expression.