Das et al. 2025 Source files for Westernblots

Das_etal2025_Source_files_WesternBlots The file contains the original uncropped images of Western blots, which will be published in "PKD2-like proteins target Chlamydomonas PKD2 to distinct subciliary regions" by Poulomi Das1, Haixia Zhou2, Nathan Chasen1, Jin Dai2, Alan Brown2, and Karl Lechtreck1# 1Department of Cellular Biology, University of Georgia, Athens, GA 30602 and 2Department of Biological Chemistry & Molecular Pharmacology, Harvard Medical School, Boston, MA 02115 Dasetal2025_PKD2-miniT_MS_table_full Mass spectrometry analysis of control (g1) and pkd2 PKD2-miniT-TG samples (Mini). For each strain, eight samples were analyzed, of which seven were axonemal fractions (AXO), including two obtained from regenerating cilia ((REGEN), and one derived from the membrane and matrix (MM) fraction. Beads, samples were digested on the beads; Elute, proteins were eluted from the beads prior to analysis. The mass spectrometry source data are available at PRIDE (Project accession: PXD071255; Project DOI: 10.6019/PXD071255). Dasetal2026_IP_mst3PKD2-mNG_table_full Mass spectrometry data of immunopurified eluates from control (CC-124) and mst3 PKD2-mNG cilia. Peptides representing PKD2, SIP and PPI were only detected in the experimental sample. A total of 150 distinct proteins were identified; peptides for other PKD2-like 5TMH proteins were not detected.