Effect of V3, MPER and IDR peptides on ID50 neutralization titers of the cross-neutralizing plasma in depletion and competition experiments.

The table describes the change in ID50 neutralization titers of the three cross-neutralizing plasma CNP (AIIMS206, AIIMS239 and AIIMS249) against 5 subtype-C and 3 subtype-B viruses upon depletion and competition with V3, MPER and IDR specific peptides.
aID50; The numerical values are the ID50 neutralization titers of CNP after depletion (‘D’) (passing plasma over antigen coated ELISA plates @10 µg/ml) and competition ‘+’ (incubating CNP with specific peptides @20 µg/ml) along with untreated and mock (passed CNP on uncoated ELISA plates) controls.
bID50 fold decrease; Plasma ID50 fold decrease for depletion assay was calculated as, ID50 with mock depleted plasma/ID50 with V3, MPER and IDR depleted plasma, and for competition assay as ID50 with untreated plasma/ID50 with V3, MPER and IDR treated plasma. The more than threefold change in ID50 neutralization titer was arbitrarily taken as positive. The CNP AIIMS206 and AIIMS239 showed dependence on MPER (Bold) directed antibodies especially with peptide competition while V3 and IDR showed minimal effect on neutralization as compared to untreated and mock plasma controls.