In-Depth Characterization of PEGylated Liposomes: Using AF4 and HPLC-CAD in Tandem as a Strategy for Composition Assessment and Post-Insertion Optimization

Given the importance of lipid nanoparticles in the therapeutic field, as in the case of vaccines against SARS-CoV-2, this study proposes a comprehensive workflow to overcome critical issues related to pegylated liposome synthesis and quality control. The aim is to assist the scientific community in optimizing pharmaceutical formulations to ensure efficacy and safety.