Table_2_Immunogenomic Profiling Demonstrate AC003092.1 as an Immune-Related eRNA in Glioblastoma Multiforme.DOCX

Enhancer RNAs, a type of long non-coding RNAs (lncRNAs), play a critical role in the occurrence and development of glioma. RNA-seq data from 161 glioblastoma multiforme (GBM) samples were acquired from The Cancer Genome Atlas database. Then, 70 eRNAs were identified as prognosis-related genes, which had significant relations with overall survival (log-rank test, p < 0.05). AC003092.1 was demonstrated as an immune-related eRNA by functional enrichment analysis. We divided samples into two groups based on AC003092.1 expression: AC003092.1 High (AC003092.1_H) and AC003092.1 Low (AC003092.1_L) and systematically analyzed the influence of AC003092.1 on the immune microenvironment by single-sample gene-set enrichment analysis and CIBERSORTx. We quantified AC003092.1 and TFPI2 levels in 11 high-grade gliomas, 5 low-grade gliomas, and 7 GBM cell lines. Our study indicates that AC003092.1 is related to glioma-immunosuppressive microenvironment, and these results offer innovative sights into GBM immune therapy.

增强型RNA，作为一种长非编码RNA（lncRNA），在胶质瘤的发生与发展中扮演着至关重要的角色。本研究从癌症基因组图谱数据库中收集了161例胶质母细胞瘤（GBM）样本的RNA测序数据。随后，我们鉴定出70个与预后相关的eRNA基因，这些基因与总生存期（log-rank检验，p < 0.05）具有显著相关性。通过功能富集分析，AC003092.1被证实为一种免疫相关eRNA。基于AC003092.1的表达水平，我们将样本分为两组：高表达组（AC003092.1_H）和低表达组（AC003092.1_L），并通过对AC003092.1的免疫微环境影响进行单样本基因集富集分析和CIBERSORTx的系统分析。我们还对11例高级别胶质瘤、5例低级别胶质瘤和7个GBM细胞系的AC003092.1和TFPI2水平进行了量化。本研究表明，AC003092.1与胶质瘤免疫抑制微环境相关，这些结果为胶质母细胞瘤的免疫治疗提供了创新性的视角。