Crystal structure of the P450 BM3 heme domain mutant F87V/T268I in complex with N-imidazolyl-pentanoyl-L-phenylalanine,ethylbenzene and hydroxylamine

Crystal structure of the P450 BM3 heme domain mutant F87V/T268I in complex with N-imidazolyl-pentanoyl-L-phenylalanine,ethylbenzene and hydroxylamine Descriptor: (2~{S})-2-(5-imidazol-1-ylpentanoylamino)-3-phenyl-propanoic acid, Bifunctional cytochrome P450/NADPH--P450 reductase, HYDROXYAMINE, ... Authors: Dong, S, Chen, J, Jiang, Y, Cong, Z, Feng, Y. Deposit date: 2022-07-04 Release date: 2023-05-24 Last modified: 2023-11-29 Method: X-RAY DIFFRACTION (1.472 Å) Cite: Regiodivergent and Enantioselective Hydroxylation of C-H bonds by Synergistic Use of Protein Engineering and Exogenous Dual-Functional Small Molecules. Angew.Chem.Int.Ed.Engl., 62, 2023

P450 BM3血红素结构域突变体F87V/T268I与N-咪唑基戊酰-L-苯丙氨酸（N-imidazolyl-pentanoyl-L-phenylalanine）、乙苯（ethylbenzene）及羟胺（hydroxylamine）形成的复合物的晶体结构。描述项：(2S)-2-(5-咪唑-1-基戊酰氨基)-3-苯基丙酸、双功能细胞色素P450/NADPH-细胞色素P450还原酶、羟胺。作者：Dong S、Chen J、Jiang Y、Cong Z、Feng Y。提交日期：2022年7月4日；发布日期：2023年5月24日；最后修改日期：2023年11月29日。测试方法：X射线衍射（1.472 Å）。引用：《通过协同利用蛋白质工程与外源性双功能小分子实现C-H键的区域发散性与对映选择性羟化反应》，Angew.Chem.Int.Ed.Engl., 62, 2023