Mus musculus

C57BL/6 mice received lethal irradiation (n=5) followed by intravenous injection of 10,000 CD45.1+ hematopoietic stem and progenitor cells (HSPC) or sublethal irradiation (n=5) followed by intrathymic injection of 10,000 CD45.1+ HSPC. Lin- CD45.1+ donor cells were isolated six months later from the bone marrow or the thymus and processed for single cell sequencing using the CITE-seq workflow. The 10 samples (5 bone marrow and 5 thymus samples) were labeled with hash tag oligonucleotides, pooled and the multiplexed sample was processed for library creation (HTO, ADT, GEX) and single cell sequencing. HTO, ADT and GEX libraries were created using the 10xGenomic platform. TotalSeqC antibodies for surface protein labeling included anti-c-kit, anti-Sca-1, anti-CD48, and anti-CD150-specific antibodies. Single cell sequencing (2x50) was performed using the Illumina NovaSeq X Plus system.