Increase in competence levels of tested strains using SMM culture medium.
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afold induction of competence compared to the control strain containing the empty pLOSS* vector and grown with 200 µM IPTG.
bIn a typical experiment using SMM medium and 200 µM IPTG, the 168-pLK, NCIB 3610-pLK and JW49-pLK cultures contained ∼3.5*108 viable cells/ml at the time of plating (OD600 0.65), and when using pHY300plk plasmid as donor DNA, approximately 40.0*104 (168+ pLK) 3.35*104 (NCIB3610 + pLK) and 0.47*104 (JW49 + pLK) transformants/ml were obtained.
- = not tested.
创建时间：
2013-02-21
